Orthopedics Research Today is a free monthly online journal that collates and summarizes the latest research about Orthopedics, including details on chronic injuries, muscoskeletal disorders, surgery, reconstruction.

The use of newly developed real-time PCR for the rapid identification of bacteria in culture-negative osteomyelitis.

Kobayashi N, Bauer TW, Sakai H, Togawa D, Lieberman IH, Fujishiro T, Procop GW

Department of Anatomic Pathology L25, The Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, OH 44195, USA.

We report a case of a culture-negative osteomyelitis in which our newly developed real-time polymerase chain reaction (PCR) could differentiate Staphylococcus aureus from Staphylococcus epidermidis. This is the first report that described the application of this novel assay to an orthopedics clinical sample. This assay may be useful for other clinical culture-negative cases in a combination with a broad-spectrum assay as a rapid microorganism identification method.

Published 22 December 2006 in Joint Bone Spine, 73(6): 745-7.
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